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. 2021 Dec 1;12(6):2934–2949. doi: 10.1016/j.apsb.2021.11.020

Figure 2.

Figure 2

Internalization, cytotoxicity and PD-L1 inhibition effect of JQ-1@PSNs-R in vitro. (A) Internalization of PNs-S, PSNs-S and PSNs-R on B16F10 cells and DC2.4. (B) Relative uptake efficiency of PNs-S, PSNs-S and PSNs-R on B16F10 cells at 4 or 37 °C with amiloride, methyl-β-cyclodextrin or chlorpromazine. (C) Diagrammatic sketch about investigating the interaction between B16F10 cells and PSNs. (D) The quantification results of B16F10 cells that adhered to Petri dish, PSNs-S-coated dish and PSNs-R-coated dish. (E) Cytotoxicity of free JQ-1 (15, 31, 62, 125 μg/mL), SNs-R (60, 120, 250, 500 μg/mL) and JQ-1@PSNs-R (54, 112, 225, 453 μg/mL). (F) The PD-L1 inhibition effects of free JQ-1 (30 μg/mL) and JQ-1@PSNs-R (108 μg/mL) in B16F10 cells. (G) LDH results showed the cytotoxicity of SNs-R, PSNs-R, laser and PSNs-R + L in B16F10 cells; (H) Confocal fluorescence microscopy images of live (green) and dead (red) B16F10 cells after photothermal therapy. Propidium iodide and fluorescein diacetate were used to stain dead and live cells. Scale bar = 10 μm. Data were mean ± SEM, n = 3–5 and analyzed by one-way ANOVA. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 and ∗∗∗∗P < 0.0001.