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. 2022 Feb 22;12(6):2923–2933. doi: 10.1016/j.apsb.2022.02.013

Figure 2.

Figure 2

Functional evolutionary analysis of TwCPS3, TwCPS5, and TwCPS6. (A) Sequence identity of TwCPS3, TwCPS5, and TwCPS6 at the amino acid level. (B) GC–MS analysis of the products of TwCPS3, TwCPS5, TwCPS6, and their mutants. Number 1 represents ent-CPP, number 2 represents ent-8-hydroxy-CPP, and number 3 represents KPP. (C) Four active-site residues differ between TwCPS3, TwCPS5, and TwCPS6. Pink cartoons represent the protein model of TwCPS3, yellow sticks represent the substrate GGPP, and pink sticks represent the four active site residues. (D) Sequence alignment of TwCPS3, TwCPS5, and TwCPS6.