Table 5.
List of the Antibodies Used for the Flow Cytometry
| Marker | Antibody | Cell Type |
|---|---|---|
| Phenotype panel | ||
| AARD | AARD | Viability |
| CD45 | CD45-APCH7 | Lymphocytes |
| CD3 | CD3-PerCpCy55 | T cells |
| CD4 | CD4-APC | CD4+ T cells |
| CD8 | CD8-BV650 | CD8+ T cells |
| FoxP3 | FoxP3-A488 | Treg |
| CD25 | CD25-PECF594 | Treg |
| TCRgd | TCRgd-BV711 | γδ T cells |
| HLADR | HLADR-BV421 | Activation |
| CD38 | CD38-PE | Activation |
| CD117 | CD117-BV786 | ILCs |
| NKp44 | NKp44-BV605 | ILC3 |
| CD45RO | CD45RO-PECy7 | Memory |
| Functional panel | ||
| AARD | AARD | Viability |
| CD45 | CD45-APC-Cy7 | Lymphocytes |
| CD3 | CD3-PerCpCy55 | T cells |
| CD4 | CD4-APC | CD4+ T cells |
| CD8 | CD8-BV650 | CD8+ T cells |
| FoxP3 | FoxP3-A488 | Treg |
| CD127 | CD127-PE-Dazzle594 | Low in Tregs |
| TCRVS2 | TCRVS2-BV711 | Vd2 T cells |
| NKp44 | NKp44-BV605 | ILC3 |
| CD117 | CD117-BV786 | ILC3 |
| TNF-α | TNFa-A700 | Cytokine |
| IFNγ | IFNg-PE-Cy7 | Cytokine |
| IL-17 | IL17-BV421 | Cytokine |
| IL-22 | IL22-PE | Cytokine |
Abbreviations: APC, allophycocyanin; PE, phycoerythrin; Treg, regulatory T cell.