Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 8;119(24):e2200200119. doi: 10.1073/pnas.2200200119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

PMC Copyright notice

Fig. 2. — Cytotoxicity of the recombinant IT in breast cancer cells. (A) Structural domains of the IT. Cytotoxicity of HB21(Fv)-PE40 was tested by the MTS assay in (B) MCF7, ER+/PR+ cell line; (C) BT474, ER+/PR+/HER2+ cell line; (D) the SUM225, ER−/PR−/HER2+ DCIS cell line; and (E) MDA-MB-231, ER−/PR−/HER2− or “triple negative” breast cancer cells. Representative graphs are shown. Each assay contained four replicates for each data point, and the assays were repeated 3 times. (F) The IC₅₀ (ng/mL) of HB21(Fv)-PE40 in each of the four breast cancer cell lines is tabulated. The IC₅₀ of MCF7 was the lowest among the four cell lines tested at 0.002 ng/mL, and the IC₅₀ of SUM225 was the highest (sevenfold higher than MCF7) at 0.158 ng/mL.