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. 2000 Aug;66(8):3277–3282. doi: 10.1128/aem.66.8.3277-3282.2000

TABLE 3.

Sensitivities of antigens detected by MAbs 12C9 and 18E2 to enzyme or chemical treatments as determined by ELISA

Treatment of crude fungal extract % Relative bindinga
MAb 12C9 MAb 18E2
None 100 100
PBS alone 98 97
Proteinase K 3 3
Pronase E 2 2
Acetate buffer alone 97 91
Periodate 57 81
a

Results, which correspond to the means of triplicate determinations, are expressed as the binding relative to that of the control performed in the absence of any treatment of the antigens.