TABLE 3.
Sensitivities of antigens detected by MAbs 12C9 and 18E2 to enzyme or chemical treatments as determined by ELISA
Treatment of crude fungal extract | % Relative bindinga
|
|
---|---|---|
MAb 12C9 | MAb 18E2 | |
None | 100 | 100 |
PBS alone | 98 | 97 |
Proteinase K | 3 | 3 |
Pronase E | 2 | 2 |
Acetate buffer alone | 97 | 91 |
Periodate | 57 | 81 |
Results, which correspond to the means of triplicate determinations, are expressed as the binding relative to that of the control performed in the absence of any treatment of the antigens.