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. 2022 Jun 7;119(24):e2103615119. doi: 10.1073/pnas.2103615119

Fig. 5.

Fig. 5.

Abrogated induction of Hh signaling markers in Myf5-Arl3−/− mice after treadmill training (T), mechanical stimulation, and stretch. (A) Relative expression of Gli1 and Ptch1 expression in TA in Myf5-Arl3−/− mice after treadmill training (T). (B) Relative expression of Gli1 and Ptch1 in myoblasts derived from WT and Myf5-Arl3−/− mice after mechanical stimulation (M) in vitro using real-time PCR. (C) Relative expression of Gli1 and Ptch1 in myoblasts derived from WT and Myf5-Arl3−/− mice after cyclic mechanical stretch (Stretch) stimulation in vitro using real-time PCR. (D) Real-time PCR analysis of Gli1 and Ptch1 expression in myoblasts derived from WT mice and treated with or without Vismodegib (Vis). (E) Real-time PCR analysis of Gli1 and Ptch1 expression in myoblasts derived from WT mice and treated with conditioned medium (CM). (One-way ANOVA with Tukey’s multiple comparisons tests, *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant.)