Fig. 2.

Timing of internal tendon cuticle breakage in TcCDA1- and TcCDA2a-deficient insects. Cryosections of legs dissected from P4, EP5, and LP5 pupae that had been injected with dsTcVer (A–C), dsTcCda1 (D–F), dsTcCda2a (G–I), or dsTcCda2b (J–L) on day 0 pupal stage were incubated with FITC-CBD, Alexa Fluor 546-phalloidin, and TO-PRO3 to stain chitin (green), actin (red), and nuclei (blue), respectively. Yellow dotted line box indicates the breakage point in internal tendon cuticle. MF, muscle fiber; PC, pupal cuticle.