Fig. 5.
Increased IL-1β production from caspase-8–deficient BMDM is responsible for enhanced T cell activation. (A) Schematic diagram of the experimental design. BMDM or mixed glia were cocultured with Th1 polarized 2D2 T cells in the presence or absence of MOG35–55. (B) Analysis of IFN-γ production in cocultures of 2D2 T cells with mixed glia or with BMDM 24 h after MOG peptide treatment. Mixed glia cultures, n = 4 biological replicates per genotype; BMDM, n = 3 biological replicates per genotype. (C) Inhibition of IL-1β/IL-1R signaling with antagonist IL-1RA (0.5 μg/mL) abolished exacerbated cytokine production by C8KOLysM cocultures treated with antigen for 48 h. WT, n = 6; C8KOLysM, n = 5. *P < 0.05; **P < 0.0.1; ***P < 0.001; ****P < 0.0001.