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. 2022 Jun 6;119(24):e2113872119. doi: 10.1073/pnas.2113872119

Fig. 6.

Fig. 6.

ZBP1 promotes LPS-induced septic shock and poly(I:C)-induced inflammation. (A and B) Mouse body temperature (A) and survival (B) over time in B6, Trif−/−, and Zbp1−/− mice after IP injection with 25 mg/kg LPS. (C–E) TNF, IFNβ, and IL-1β protein levels in the (C) serum, (D) spleen, and (E) heart of B6, Trif−/−, and Zbp1−/− mice injected IP with 25 mg/kg LPS or i.v. with 15 mg/kg poly (I:C) 4 h after injection. (F) Relative LDH levels in the serum of B6, Trif−/−, and Zbp1−/− mice injected IP with 25 mg/kg LPS or i.v. with 15 mg/kg poly (I:C) 4 h after injection. For ELISA and LDH assays, data points indicate individual mice tested (B6 PBS, n = 10; Trif−/− PBS, n = 10; Zbp1−/− PBS, n = 10; B6 LPS, n = 10; Trif−/− LPS, n = 10; Zbp1−/− LPS, n = 10; B6 poly(I:C), n = 5; Trif−/− poly(I:C), n = 5; Zbp1−/− poly(I:C), n = 5). (G) Levels of full-length and cleaved CASP8, CASP3, CASP7, and GSDMD in the spleen of B6 and Zbp1−/− mice injected IP with 25 mg/kg LPS or PBS. Data from immunoblots are representative of three or more independent experiments. Unless otherwise indicated, blots were singly or doubly probed to ensure highest quality. All blots from a specific panel are run from a single set of lysates that were identically handled. Log-rank (Mantel-Cox) test was used for comparison of survival curves. ANOVA was used for comparison between groups: ns, nonsignificant (P > 0.05); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.