Fig. 7.

Both the agonist carbachol and the antagonist pirenzepine favor monomerization of M₁ in neuronal cultures of M₁-mEGFP–expressing mice. Results from FIF spectrometry analysis of neuronal cultures from M₁-mEGFP–expressing mice as detailed in Fig. 6 are presented as monomers, dimers, and oligomers for data sets encompassing receptor density across the range of 25 to 55 molecules/μm². *P < 0.05, ***P < 0.001, ****P < 0.0001. One way ANOVA followed by Tukey’s multiple comparisons test. ns, not significant.