Fig. 4.

RYK acts as a survival factor and antiinflammatory modulator during lung maturation and homeostasis. (A) Diagram indicating the time points of tamoxifen injections and tissue collection for the early postnatal Gli1-Ryk^cKO mice. (B) H&E staining of P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. (C) High magnification of H&E-stained P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. (D) IsoB4 staining (immune cells) and F4/80 immunostaining (alveolar and interstitial macrophages) in P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. (E) Immunostaining for CD45 (hematopoietic cells) in P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. (F) TUNEL staining and E-cadherin immunostaining in P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. Arrowheads point to TUNEL-positive cells. (G) Immunostaining for PECAM-1 in P10 Gli1-Ryk^WT (n = 6) and Gli1-Ryk^cKO (n = 6) lung sections. High-magnification image of the areas in the dashed boxes is shown on the Right. (H) Immunostaining for RYK in the lungs of healthy controls (n = 5) and pneumonitis patients (n = 10). High-magnification image of the areas in the dashed boxes is shown on the Bottom. (I) Immunostaining for CCL2 in the lungs of healthy controls and pneumonitis patients. Abbreviations: al, alveoli; br, bronchiole; bv, blood vessel; and il, inflammatory lesion. (Scale bars: 1 mm for B; 200 μm for H, Top; 50 μm for H, Bottom, and I; and 30 μm for D, F, and G.)