FIG. 1.
Schematic diagram of JMP3 construction. (A) pHSS6 was introduced into pINA1067 at the NotI site, giving rise to JMP1. pBR322 and the XPR2 promoter were replaced by a polylinker, resulting in the defective JMP3 vector. (B) The sequences of the polylinker and the unique cloning sites present are shown. The expression cassette is liberated by NotI or SalI-EcoRI digestion (boxed). Genes conferring tetracycline (TetR) and kanamycin (KanR) resistance are indicated by an arrow. ura3d4 is a modified version of the Y. lipolytica URA3 gene (20). Arrows indicate direction of transcription. Zeta regions are indicated by an open box.