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Fig. 5. (A) and (B) MDA-MB-231 cells were treated with various concentrations of PAA@DOX, PAA-POSS@DOX and free DOX for 24 h and 48 h. Cell viability was measured by the MTT assay. (C) MDA-MB-231 cells were treated with 20 μg ml−1 of PAA@DOX, PAA-POSS@DOX and free DOX for 24 h. Cell lysates were analyzed by immunoblotting.