KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Donkey anti-Goat IgG Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A32758 |
| Donkey anti-Rabbit IgG H&L Alexa Fluor 488 | Abcam | Cat# ab150065 |
| Goat polyclonal anti-S. pyogenes carbohydrate | Abcam | Cat# ab9191 |
| Rabbit polyclonal anti-caspase-1 | GeneTex | Cat# GTX14368 |
| Bacterial and Virus Strains | ||
| Streptococcus pyogenes M1T1 strain 5448 | Kansal et al., 2000 | Accession:CP008776 |
| XL-10 Gold | Agilent Technologies | Cat# 200314 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Carboxyfluorescein diacetate succinimidyl ester | Invitrogen | Cat# C1157 |
| Mutanolysin | Sigma Aldrich | Cat# M9901 |
| Staurosporine | Sigma Aldrich | Cat# S6942 |
| Streptozotocin | Adipogen | Cat# 50-464-382 |
| Critical Commercial Assays | ||
| ATP-bioluminescent assay Kinshiro | TOYO B-Net | Cat# LL100-1 |
| Click-iT Plus TUNEL Assay Kit (Alexa Fluor 488) | Thermo Fisher Scientific | Cat# C10617 |
| Glucose Colorimetric Assay Kit II | Biovision | Cat# K686 |
| LIVE/DEAD Viability/Cytotoxicity Kit | Thermo Fisher Scientific | Cat# L3224 |
| Deposited Data | ||
| Raw data files for RNA-sequencing | DDBJ SRA | DRA009112 |
| Experimental Models: Cell Lines | ||
| HEK-Blue IL-1β reporter cells | InvivoGen | Cat# hkb-il1bv2 |
| Human: HaCaT cells | Boukamp et al., 1988 | Human: HaCaT cells |
| Experimental Models: Organisms/Strains | ||
| Filaggrin-null mouse strain (B6.Cg-Flg<tm1>) | RIKEN BRC | RBRC05850 |
| Oligonucleotides | ||
| See Table S2 | ||
| Recombinant DNA | ||
| Plasmid: pSET4-ArcAKO | This paper | N/A |
| Plasmid: pSET4-5’Flag-CovR | This paper | N/A |
| Plasmid: pQE30_arcA | This paper | N/A |
| Software and Algorithms | ||
| CLC Genomics workbench v. 9.5.2 | Software | https://digitalinsights.qiagen.com/ja/qiagen-clc-genomics-workbench/ |
| ClustVis | Software | https://biit.cs.ut.ee/clustvis/ |
| GraphPad Prism7 | Software | https://www.graphpad.com/scientific-software/prism/ |
| iDEP.91 | Software | http://bioinformatics.sdstate.edu/idep/ |
| ImageJ | Software | https://imagej.nih.gov/ij/ |
| ImageQuant software | Software | https://www.cytivalifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110 |
| Other | ||
| AimStrip Plus blood glucose meter kit | Germaine Labs | Cat# 37355 |
| Citrate buffer | Bioworld | Cat# 40320056-2 |
| cOmplete, EDTA (+) Protease Inhibitor Cocktail | Roche Molecular Diagnostic | Cat# 11697498001 |
| Lysing Matrix B | Qbiogene | Cat# FAS-210 |
| lysing Matrix D | Qbiogene | Cat# FAS-220 |
| Nunc Lab-Tek II Chamber Slide System | Thermo Fisher Scientific | Cat# 154534 |
| Phenol red broth | Sigma Aldrich | Cat# P8976 |
| Phos-tag SuperSep™ Phos-tag™ Gels | Wako Pure Chemical Industries | Cat# 195-17991 |
| PhosSTOP™ | Roche Molecular Diagnostic | Cat# 4906845001 |
| Quanti-Blue reagent | Invivogen | Cat# rep-qbs |
| RNAprotect Animal Blood Tubes | Qiagen | Cat# 76544 |
| RNAprotect Bacteria Reagent | Qiagen | Cat# 76506 |
| RNeasy Mini Kit | Qiagen | Cat# 74104 |
| rRNA removal using a Ribo-Zero rRNA removal kit | Illumina Inc | Cat# 20040525 |
| Superscript VILO cDNA synthesis kit | Thermo Fisher Scientific | Cat# 11756050 |
| SYBR green RT-PCR master mix kit | Toyobo | Cat# QPK-201 |
| Todd-Hewitt broth | BD Biosciences | Cat# 249240 |
| TruSeq RNA Sample Prep kit, v2 | Illumina Inc | Cat# RS-122 |
| Yeast extract | BD Biosciences | Cat# 212750 |