Table 1.

Primer sequences used in qPCR. Annealing temperatures for all primer pairs were 60 °C. TNF-α - Tumor necrosis factor α, IL - interleukin, UCP1 - uncoupling protein 1, CPT1 - carnitine palmitoyltransferase 1, PGC1-α -peroxisome proliferator γ coactivator 1-α.

Primer name	Forward primer 5′- > 3′	Reverse primer 5′- > 3′
18S	CCAAGATCCAACTACGAGCTT	GGCCCTGTAATTGGAATGAGTC
p16INK4a	GGGTCGGGTAGAGGAGGTG	GCCTCCGACCGTAACTATTCG
p21Cip1	TATGGGGCTGGGAGTAGTTG	ACATTTGGAGAGCTCCCGTC
TNF-α	CCCAGGGACCTCTCTCTAATC	ATGGGCTACAGGCTTGTCACT
IL-6	GTGCCTCTTTGCTGCTTTCAC	GGTACATCCTCGACGGCATCT
Il-1β	AAACAGATGAAGTGCTCCTTCCAGG	TGGAGAACACCACTTGTTGCTCCA
UCP1	CCGGCTCCAGGTCCAAG	CCGATCCTGAGAGAGGCG
CPT1a	GAGAGGAGACAGACACCATCCA	CTGAGGATCCGAGGTATTGTCC
PGC1-α	CTCTGGAACTGCAGGCCTAACT	TGGAGTTATTGCCTTGTGTACCAG