Fig. 2. FLIM shows the difference in kinetics and the nature of the inclusions formed by polyQ versus α-synuclein. Shown are the head regions of C. elegans with expression in the muscle cells of YFP (control), 40 glutamines tagged with YFP (Q40) and α-synuclein tagged with YFP (α-syn). Amyloid formation is accompanied by a reduction in the fluorescence lifetime (blue-green colours). Q40 forms amyloid-like inclusions directly, whereas α-synuclein accumulates in inclusions that are not amyloid-like until the worms have reached old age. Based on ref. 118.