Figure 2. Cuff preparation with various sizes of angio-catheter and rib-back surgical blade #11.

The size of the angio-catheter chosen for the cuff is determined by the cuff size guide (Table 1) that takes into account the rat’s body weight and whether the cuff is for the pulmonary artery (PA), bronchus (Br), or pulmonary vein (PV). The angio-catheters (A) 20 G, (B) 18 G, (C) 16 G, (D) 14 G, or (E) 12 G are cut with a (F) rib-back surgical blade #11 as described in the protocol, and stored in saline until needed. (G) The cuff body length is 2 mm and a 1 mm X 1 mm tab (width x height) is left at the top of the cuff body for handling of the cuff body.