Fig. 3.

Synthetic peptide R⁷-G-Aβ_25-35induces cell death in a CASPASE-3 (CASP-3)- and reactive oxygen species-independent mechanism in Jurkat cells

(A) Representative histograms show the DCF ⁺ mean percentage in Jurkat cells treated with R⁷-G-Aβ_25-35 peptide (200 μM) for 0, 4, 8, and 24 h. (B) Representative histograms show the CASP-3⁺ mean percentage of Jurkat cells treated with R⁷-G-Aβ_25-35 (200 μM) peptide for 0, 4, 8, and 24 h. (C) Representative histograms show the DiOC₍₆₎3^low mean percentage of Jurkat cells untreated, or with NSCI (10 μM) or NAC (5 mM) only for 24 h. (D) Representative histograms show the DiOC₆(3)^low mean percentage of Jurkat cells treated with R⁷-G-Aβ_25-35 peptide (200 μM) only or with NSCI (10 μM) or with NAC (5 mM) for 24 h. The numbers represent the mean percentage of three independent experiments. *p<0.05; **p<0.01; ***p<0.001.