TABLE 1.
Most important bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| Pseudomonas putida UWC3 | Rifr ILV− | 30 |
| Ralstonia eutropha JMP228 | Rifr | 16 |
| Ralstonia eutropha JMP228n | Rifr Nxr | 52 |
| Plasmids | ||
| pUT-mini-Tn5 KmlacZ | Kmr LacZ+ | 13 |
| pEMT1 | Tfd+ | 52 |
| pJP4 | Tfd+ 3-CBA+ Hgr | 16 |
| pEMT1::lacZ | Tfd+ Kmr LacZ+ | This study |
| pJP4::lacZ | Tfd+ 3-CBA+ Hgr Kmr LacZ+ | This study |
a
Rifr, Nxr, Kmr, and Hgr, resistance to rifampin, nalidixic acid, kanamycin, and mercury, respectively; ILV−, auxotrophic for isoleucine, leucine, and valine; Tfd+ and 3-CBA+, abilities to degrade 2,4-D and 3-chlorobenzoate, respectively; LacZ+, β-galactosidase activity.