Extended Data Fig. 10 |. Histology from DREADD and CNO experiments.

a, Top, histology from the cohort of rats with AAV1-hDlx-GiDREADD-dTomato-Fishell injected into lateral septum. Bottom, example cross-correlograms between SPW-R and glucose fluctuations on vehicle (blue) and CNO (black) days. b, Top, histology from the cohort of rats with AAV2-hSyn-hM4D(Gi)-mCherry injected into medial septum. Bottom, example cross-correlograms between SPW-R and glucose fluctuations on vehicle (blue) and CNO (black) days. c, Comparison of effects for individual rats across vehicle versus CNO days for each cohort. d, P values (two-way t-test) for each part of the cross-correlograms shown in e when comparing within hM4Di rats (red; CNO versus vehicle) or across cohorts of rats (blue; CNO–hM4Di vs CNO–control). e, Ten colleagues who were not involved with these experiments were provided histology from all 9 rats (6 lateral septum; 3 medial septum) and asked to rate the degree of viral transfection in each subregion of the septum. Each rat had three images taken along the anterior–posterior axis and the individuals were blinded as to which virus had been injected and the effects of CNO on the SPW-R–glucose correlation. For each rat and subregion, the average score from these 10 individuals is shown in the table. For each subregion, the correlation between these scores and the effect of CNO was calculated and is shown in bold below. Red values had a P values < 0.05. f, Scatter plot of the SPW-R–glucose correlation after CNO injection (x axis) and the average expression score given by these rates (y axis) for the dorsal lateral septum. g, Scatter plot of the SPW-R–glucose correlation after CNO injection (x axis) and the average expression score given by these rates (y axis) for the medial septum.