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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Nat Methods. 2022 May 11;19(5):613–619. doi: 10.1038/s41592-022-01468-5

Extended Data Figure 5 |. Imaging of immunostained and endogenously fluorescent CUBIC-cleared mouse brains.

Extended Data Figure 5 |

Hybrid OTLS imaging of whole mouse brains. (a-c) with endogenously preserved GCaMP6s fluorescence, and immunostained with (d) anti-ChAT antibody + SYTOX-G or (e) anti-Parvalbumin (PV) antibody + SYTOX-G by CUBIC-HistoVision. Scale bar lengths are as follows: (a-b) 1 mm, (c) 10 μm, (d-e) 2 mm. The imaging data in (a-e) was acquired from in a single experiment.