NSCs medium

Reagent	Final concentration	Amount
DMEM/F-12	n/a	46.98 mL
B27 (50×)	1×	1 mL
N2 (100×)	1×	500 μL
GlutaMAX (100×)	1×	500 μL
MEM NNEA (100×)	1×	500 μL
Pen/Strep (100×)	1×	500 μL
EGF (100 μg/mL)	20 ng/mL	10 μL
bFGF (100 μg/mL)	20 ng/mL	10 μL
Total	n/a	50 mL