Neuronal differentiation medium

Reagent	Final concentration	Amount
Neurobasal	n/a	47.47 mL
B27 (50×)	1×	1 mL
GlutaMAX (100×)	1×	500 μL
MEM NNEA (100×)	1×	500 μL
Pen/Strep (100×)	1×	500 μL
Ascorbic Acid (200 μg/mL)	35.2 ng/mL	8.8 μL
GDNF (100 μg/mL)	20 ng/mL	10 μL
BDNF (100 μg/mL)	20 ng/mL	10 μL
Total	n/a	50 mL