Fig. 2. (A–C) 3D fluorescence images at the Z-section and along with XZ cross-sectional analysis of siRNA@PT-ZIF-8 based on green fluorescence of FAM-siRNA, red fluorescence of IR780-1, and their merged fluorescence of CLSM. Scale bar: 100 μm. (D) Fluorescence spectra of IR780-1 and the supernatant (abbreviated as “s”) of siRNA@PT-ZIF-8. (E) Fluorescence spectrum of FAM-siRNA in different groups. (F) The fluorescence intensity of FAM-siRNA released from FAM-siRNA@PT-ZIF-8 in pH 5.5 or 7.4 PBS solution (n = 3, mean SD). (G) Photothermal curves of different concentrations of siRNA@PT-ZIF-8 under laser irradiation. (H) The corresponding infrared thermography images. (I) Temperature profiles of siRNA@PT-ZIF-8 and IR780-1 irradiated by using an 808 nm laser at 2 W cm−2, followed by natural cooling with laser turn-off.
