Fig. 3. Cellular uptake analysis of different agents in 4T1 cells. (A) CLSM images and (C) flow cytometry analysis of cellular uptake of IR780-1, siRNA@PT-ZIF-8, and Apt/siRNA@PT-ZIF-8 at a concentration of 50 μg mL−1 for 12 h, respectively. Scale bar: 50 μm. (B) The average fluorescence intensity of IR780-1 in 4T1 cells after incubation of 4T1 with different agents for 12 h. (D) Western blot assay of the HSP70 protein from differently treated 4T1 cells: (1) Apt/siRNA@PT-ZIF-8 + NIR, (2) PBS, (3) PBS + NIR, (4) siRNA, (5) PT-ZIF-8, and (6) PT-ZIF-8 + NIR.
