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. 2022 Jun 22;19:22. doi: 10.1186/s12014-022-09360-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Oxidations changing with aging in male rat cohort. A Experimental flow chart of rat cohort. B and C open-search, unsupervised cluster analyses of both all identified modifications and all oxidations state oxidations in old group is obviously different from the low age groups. D 15 oxidations show an upwards trend with aging. E 8 oxidations are differential in old from others, and they are almost linear increasing with aging using two tailed tests. Data are presented as mean ± SD. As the number of labels increases, the significance increases. (*p <0.05, **p < 0.01, ***p < 0.005, ****p < 0.001)