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. 2022 Jun 22;21:133. doi: 10.1186/s12943-022-01596-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Trajectory analysis and function enrichment of the UCOGCP-specific EMT cells. A, Major clusters of the UCOGCP-specific EMT cells shown in UMAP. B, Top three markers of each cluster obtained from “FindAllMarkers” function from Seurat package (4.0.4) shown in dot plot. C, Expression pattern of interested gene markers. D, Mapping of cluster 15 in Fig. 2D. E, GO BP enrichment of cluster 0. F, Trajectory analysis by monocle 3. G, Top ten genes differentially expressed in different clusters. H, Heatmap showing gene modules co-expressed in different clusters. I, GO BP enrichment of gene modules closely related to cluster 0. Genes in module 1, 2, 5, 6, and 10 were used for GO BP enrichment