Fig. 2.

KRAB depletion abolishes CRISPR/dCas9 system repression. A GFP expression of each sgRNA (LTR2-LTR5) transduced J.Lat 10.6 cells treated with 1 µM PMA. pdCas9ko/KRAB is the sgRNA and dCas9 expression vector without the KRAB domain sequence and was used as a negative control B GFP expression of LTR2 to LTR5 J.Lat 10.6 cell clones with and without KRAB domain expression upon PMA stimulation. C GFP expression of LTR2 to LTR5 J.Lat 10.6 cell clones with and without KRAB domain expression upon IngB stimulation. The statistical analysis of A–C was performed using one-way ANOVA comparing mean of each column with mean of each other column with LTR2ko/KRAB to LTR5ko/KRAB for PMA group (**p < 0.0004; ****p < 0.0001) and for IngB group (**p < 0.0058; ***p < 0.0007; ****p < 0.0001). The statistical analysis comparing LTR4 (ns—PMA and IngB) or LTR5 (*p < 0.0302—PMA/*** p < 0.0004—IngB) with and without KRAB was performed by one-way ANOVA with multiple comparisons). The mean values of two technical measures of three independent biological experiments are shown