Table 1.

Constructs sequences, ligation sites and position to HIV-1 TSS

Vector name	sgRNA sequence	HIV-1 provirus ligation sitea	HIV-1 genome position	Position from 5′LTR TSS
pLTR1	5′CCACGTGATGAAATGCTAGG3′	3′LTR U3	9360–9379	+ 8924
pLTR2	5′CCGCCTAGCATTTCATCACG3′	3′LTR U3	9357–9376	+ 8921
pLTR3	5′TGCCTGGCTAGAAGCACAAG3′	NEF	8961–8980	+ 8525
pLTR4	5′CTGTGGATCTACCACACACA3′	5′LTR U3/3′LTR U3	45–64/9130–9149	− 391
pLTR5	5′CTACAAGGGACTTTCCGCTG3′	5′LTR U3R 3′LTR U3R	344–363/9429–9448	− 92

pLTR—expression vector containning sgRNA, the dCas9 and KRAB domain

^aSequence from subtype B group M HIV-1