Table 3.
Molecular identification of Trypanosoma species with the ITS1 gene
| No. | Sample ID | Identification of Trypanosoma sp. (accession, similarity %) | Host | Region | Sex of the tsetse fly |
|---|---|---|---|---|---|
| 1 | M10 | –* |
Human African buffalo |
Maswa Game Reserve | Female |
| 2 | M21 | Trypanosoma simiae (JN673382, 100%) |
Human African elephant |
Maswa Game Reserve | Female |
| 3 | M60 | PCR failed |
Human Common warthog Mouse |
Maswa Game Reserve | Male |
| 4 | S17 |
Trypanosoma godfreyi (JN673383, 95.3%) |
NGS failed | Serengeti National Park | Female |
| 5 | S55 |
Trypanosoma godfreyi (MK131836, 99.4%) |
African buffalo | Serengeti National Park | Male |
| 6 | T73 | PCR failed |
Human Mouse |
Tarangire National Park | Male |
PCR was performed with primers targeting the Trypanosoma-specific ITS1 gene, and conventional DNA sequencing was performed to identify Trypanosoma species
*DNA sequencing failed because of weak amplicon band