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. Author manuscript; available in PMC: 2022 Jun 22.
Published in final edited form as: Clin Sci (Lond). 2021 Mar 26;135(6):793–810. doi: 10.1042/CS20201047

Fig. 2. The AT1R mediates the effects of sPRR-His activation.

Fig. 2.

Effects of 10μM Los (A) or AT1R siRNA knockdown (B) on mRNA expression of IL-6, IL-8, VCAM-1 and ICAM-1 after sPRR-His 3-h treatment. Effect of 10μM Los on protein levels of IL-6 (C) and IL-8 (D) in the medium of HUVECs after 50nM sPRR-His treatment. (E-F) Effects of 10μM Los on sPRR-His-induced apoptosis. (E) The apoptotic populations were determined by Annexin V-FITC/PI flow cytometry analysis. (F) Quantitative analysis of the percentage of total apoptotic cells. The early apoptotic cells percentage added to the late apoptotic cells percentage equals the total apoptotic cells percentage. (G) Validation of AT1R knockdown by qRT-PCR. N=9–12 (N represents the number of samples in each group, and the repetitions of separate experiments are 3–4). Statistical analysis was performed by using one-way ANOVA with the Bonferroni test for multiple comparisons or by using unpaired Student’s t test for two comparisons. *P<0.01, compared with the CTR or Scr siRNA; #P<0.01, compared with the sPRR-His or sPRR-His+Scr siRNA. Data are mean ± SEM.