FIGURE 5.

Crocin and antibiotics reduce HTTex1 aggregation and mortality. (A) SDD-AGE and WB analysis of lysates generated from the Ex1-HD larvae treated with crocin or penicillin/streptomycin (ABX). PHP1 antibody was used to detect aggregates. Elav-Gal4 larvae were used as a negative control. For each condition, ten larvae (n = 10) were pooled and analyzed. (B) Representative confocal images of brains from Ex1-HD larvae untreated (Control) and treated with crocin or ABX, and immunolabelled with PHP1 (green), anti-Elav antibody (red), and DAPI (blue). Part (C) shows the percent of pupal survival of Ex1-HD larvae treated with crocin or ABX. The data are represented as mean ± SEM, one-way ANOVA with Tukey’s post hoc test. n = 5 independent crosses for each condition, ***p < 0.001.