Table 1.

Table to show advantages and disadvantages of zebrafish cardiac injury models.

Injury	Schematic	Advantages	Disadvantages	References
Ventricular resection		Fast recovery Common in literature Injury to all cell types	No lymphatic regeneration Less similar to human MI Technically challenging Variable injury size Open chest model	Poss, Wilson & Keating, 20024; Raya et al., 200330
Cryoinjury		Common in literature Most similar to human MI Injury to all cell types	Long recovery Technically challenging Open chest model	Chablais et al., 20115; González-Rosa et al., 20116; Schnabel et al., 20117
Genetic ablation		Cell-specific study Non-invasive Fast recovery Technically simple	Limited to single-cell type Less similar to human MI	Curado et al., 20078; Wang et al., 20119
Hypoxia/reoxygenation		Reperfusion injury Non-invasive Similar to human MI Technically simple	No localized version currently available	Parente et al., 201310
Explant culture		Fewer ethical concerns No requirement for home office approval Live imaging	Loss of circulating factors Declines in heart function Injury response from extraction	Hecker et al., 200832; Pieperhoff et al., 201434; Cao and Poss., 201635; Yip et al., 202036
Laser injury		Non-invasive Consistent injury Fewer ethical concerns No requirement for home office approval Live imaging High N-numbers	Limited to larval stages Requirement for specialized equipment	Matrone et al., 201311; Kaveh et al., 202037