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. 2021 Jun 4;118(7):1805–1820. doi: 10.1093/cvr/cvab187

Figure 7.

Figure 7

Loss of Bmp9 in mice is associated with reduced endothelin-1 (ET-1) abundance and ET-1 plays a crucial role in BMP9-induced vasoconstriction in chick chorioallantoic membrane. ET-1 plasma (A) levels in WT, Bmp9-KO, Bmp10-cKO, and DKO mice (n = 8–10 male mice/group) and lung mRNA (B) in WT, Bmp10-cKO, and DKO mice (n = 5–13 male mice/group). Mice were injected with tamoxifen at the age of 2 months and analysed at the age of 5 months. Data are presented as the mean ± SEM and were analysed using Kruskal–Wallis tests followed by Dunn’s tests. *P < 0.05, **P < 0.01 vs. WT. Quantitative analysis (C) and representative photomicrographs (D) of FITC-Dextran-injected blood vessels from chick chorioallantoic membranes treated with bosentan (0, 50, and 500 µM), in combination with BMP9 (20 nM) or not for 24 h (scale bar = 250 um). Data are presented as the means ± SEM of n = 3–6 eggs/group and were analysed using Kruskal–Wallis tests followed by Dunn’s tests. *P < 0.05 vs. vehicle treated control and ##P < 0.01 vs. the 20 nM BMP9 condition.