FIG 3.

KSHV RTA and ID2 interact. (A) Coomassie blue staining of GST (control) and GST-ID2 protein purified from E. coli. (B) Immunoblot analysis of in vitro GST pulldown (GST-PD) using GST (control) and GST-ID2 with cell extracts of 293T expressing 3xFLAG-RTA. (C) iBCBL1-3xFLAG-RTA cells were reactivated and treated with MG132 for 6 h and then fixed and permeabilized before the proximity ligation assay was performed. Cells were probed with anti-FLAG and anti-IgG control (top) or with anti-FLAG and anti-ID2 antibodies (bottom).