FIG 5.

RTA-mediated degradation of ID2 does not require host E3 ubiquitin ligases ITCH or RAUL. (A) Immunoblot analysis of iSLK cells that were transduced with lenti-ID2 and lenti-shControl or lenti-shITCH for 3 days and then treated with 1 μg/mL of Dox to induce RTA expression for 24 h. (B) Western blot analysis of iSLK cells that were treated with lenti-ID2 and lenti-shControl or lenti-shRAUL for 3 days and then induced with 1 μg/mL of Dox to express RTA for 24 h. Band intensities of ID2 were quantified relative to tubulin (loading control) and presented as a ratio relative to the Dox- negative control of each condition in panel B.