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. 2022 May 23;96(12):e00101-22. doi: 10.1128/jvi.00101-22

FIG 8.

FIG 8

ID2 diminishes TPA-induced lytic reactivation of KSHV in BCBL1 cells. (A) Immunoblot analysis of ID2 and viral proteins in lenti-GFP-, lenti-ID2-, and lenti-3xFLAG-ID2-transduced BCBL1 cells during TPA-induced KSHV reactivation. (B) RT-qPCR measurement of viral gene expression at 24 hpi and 48 hpi in samples shown in panel A. (C) Viral DNA replication measured by qPCR during KSHV reactivation in samples described in panel A. (D) Immunoblot detection of lytic viral proteins and ID2 in ID2 lenti-shRNA-transduced BCBL1 cells at 3 days postransduction. (E) RT-qPCR analysis of lytic viral gene expression in samples shown in panel D. t tests were performed between GFP-OE and ID2-OE or shControl and shID2, and degree of statistical significance is indicated as *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001 (sample of n = 3).