FIG 9.

Degradation of ID proteins by gammaherpesviral RTAs. (A) Immunoblot analysis of 293T cells after being cotransfected for 2 days with C-terminally HA-tagged ID1-ID4 and either a vector control or a 3xFLAG-tagged RTA derived from KSHV, EBV, MHV68 or RRV. (B) 293T cells were transfected with a vector control or 3xFLAG-tagged RTA from KSHV, EBV, and MHV68 for 2 days. Immunoblot detection of input fraction of cell lysates that were used for in vitro GST pulldown (GST-PD) in panel C. (C) Immunoblot analysis of in vitro GST-PD samples.