FIG 10.

Effect of GNB1 on IAV polymerase activity. (A) Effect of overexpressed GNB1 on IAV polymerase activity. The 293T cells were transfected with plasmids containing NP, PB2, PA, PB1, PoI II, pRL-TK, and Flag-GNB1 (0.5 and 1 μg) for 24 h. Then, the luciferase activity was measured, and Renilla luciferase was used as an internal control (*, P < 0.05; **, P < 0.01; one-way analysis of variance [ANOVA]). The expression level of each protein was also detected by Western blot. (B) Effect of silencing GNB1 on polymerase activity. The 293T cells were transfected siRNA targeting GNB1 (si-1, si-2) or si-NC (negative control) for 36 h, and then cells were transfected with the indicated plasmids as above. Then luciferase activity was measured, and the GNB1 protein expression level was detected using Western blot (***, P < 0.001; one-way ANOVA). (C) The effect of si-GNB1 on 293T cell viability. Cell viability was measured by CCK-8 assay at 24 h and 48 h after being transfected with siRNA targeting GNB1 (si-1 and si-2) or si-NC.