FIG 6.

EndoU-deficient IBV infection induced colocalization of N protein/double-strand (ds)RNA and TIA1 in infected cells. CEK cells were infected with parent IBV rSD strain or EndoU-deficient virus (rSD-H238A or rSD-Y334A) for 18 hpi and then immunostained. (A) Infected cells were immunostained; IBV N was detected with anti-N (green); TIA1 was detected with anti-TIA1 (red). (B) Infected cells were immunostained; IBV dsRNA was detected with J2 antibody (green); and TIA1 was detected with anti-TIA1 (red).