FIG 7.

TIA1 overexpression inhibited IBV replication. (A) TIA1 was constructed into PRK-EGFP, the plasmid was electroporated in CEK cells, and PRK-EGFP plasmid was used as a control. At 36 hpt, CEK cells were infected with IBV and the viral protein synthesis was observed via Western blot. TIA1 overexpression was evident at 48 and 60 hpt. Relative levels of the density ratio were quantified. The N protein/tubulin density ratio of the PRK-EGFP control group was set as 1 and compared with the other experimental groups. (B) Viral replication kinetics were compared in EGFP-expressing cells and TIA1-expressing cells. Data are shown as the mean ± SD of at least three independent experiments. P values were calculated using t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns = not significant.