FIG 5.

O50.loxP latency is maintained over time despite ORF50 deletion. CD19-Cre mice were infected IN with 1000 PFU of the indicated virus. Mice were sacrificed on day 42 postinfection, and spleens were collected. (A) The frequency of cells harboring MHV68 genomes was determined by limiting-dilution PCR analysis. (B) ORF50 deletion was confirmed by comparing limiting-dilution PCR analyses performed using primers specific for either the ORF50 locus or ORF73 locus. Groups of three to five mice were pooled for each infection and analysis. Results are means of two to three independent infections. Error bars represent standard error of the means.