FIG 3.
CTR1 requirement for ERV-DC14 in different species. (A) Representative immunoblot of HA-tagged CTR1 in cell lysates from CHO cells stably transduced with MLV-based LXSN retroviral vector either empty or carrying the CTR1 cDNA from human (hu), hamster (ha), mouse (m), or cat. (B) Cells from panel A were evaluated for their sensitivity to retroviral infection by EGFP lentiviral vectors pseudotyped with either the ERV-DC14-specific Env or the VSV-G protein. Data are means ± SEM from n = 3 experiments. (C) CHO cells from panel A were evaluated for FLVCR1 and CTR1 or cell surface expression by flow cytometry using RBD ligands. Numbers (× 103) indicate the delta mean fluorescence intensity of a representative experiment (n = 3).