FIG 6.

Ectodomain of CTR1 is not required for ERV-DC14 infection and binding. (A) Schematic representations of truncated CTR1 constructs. (B) CHO cells stably transduced with LXSN retroviral vector either empty or carrying the CTR1 cDNA from human (hu) or truncated human CTR1 (hu 40 to 190 and hu 69 to 190) were evaluated for their sensitivity to infection by EGFP retroviral vectors pseudotyped with either the ERV-DC14-specific Env or the VSV-G protein. Data are means ± SEM from n = 3 experiments. (C) Cells from panel B were evaluated for FLVCR1 or CTR1 cell surface expression by flow cytometry using RBD ligands. Numbers (× 10³) indicate the delta mean fluorescence intensity of a representative experiment (n = 3). (D) 293T cells transduced with retroviral LXSN either empty or carrying the CTR1 cDNA from human (hu) or truncated (hu 69 to 190) were evaluated for their sensitivity to infection by EGFP retroviral vectors pseudotyped with the ERV-DC14-specific Env. Data are means ± SEM from n = 3 experiments.