FIG 10.

Differential dependence on viral DNA replication between phase I and II reactivation. Cultures were infected with Stayput-GFP at an MOI of 7.5 PFU/cell in the absence of ACV. Following loss of GFP, cultures were reactivated with a combination of LY294002, forskolin, and heat shock in the presence of ACV (50 μM). (A to D) Late (VP16, gC, UL10) genes (A to C) were investigated at 22 h poststimuli. GFP was counted over time, and peak GFP is presented (D). n = 12 biological replicates. Normality was determined by the Kolmogorov-Smirnov test. Mann-Whitney; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The mean and SEM are shown.