TABLE 1.
Clinical responses of pigs treated with the mixture of the vaccine and Bac-Con3N IFN-α and challenged with FMDV depending on the time of challenge and baculovirus titera
| Animal | Treatment(s) | Baculovirus titer (PFU) | Time of challenge (dpv) | Mean max amt of viral RNA detected ± SD (dpc of 1st detection)b in: |
Max clinical score (dpc of 1st detection) | |
|---|---|---|---|---|---|---|
| Serum | Saliva swab | |||||
| 1 | Bac-Con3N IFN-α and vaccine | 2 × 1010 | 1 | Neg | Neg | Neg |
| 2 | Neg | Neg | Neg | |||
| 3 | Neg | Neg | Neg | |||
| 4 | Vaccine | 2.7 × 105 ± 0.2 × 105 (2) | 1.2 × 105 ± 0.2 × 105 (1) | 9 (2) | ||
| 5 | 2.5 × 105 ± 0.6 × 105 (2) | 7.8 × 104 ± 0.1 × 104 (1) | 9 (2) | |||
| 6 | 4.2 × 105 ± 0.3 × 105 (2) | 1.3 × 105 ± 0.2 × 105 (1) | 10 (2) | |||
| 7 | Bac-Con3N IFN-α and vaccine | 3 | 1.4 × 103 ± 0.03 × 103 (4) | 2.4 × 103 ± 0.2 × 103 (2) | 2 (4) | |
| 8 | 9.0 × 102 ± 0.1 × 102 (4) | 1.0 × 103 ± 0.3 × 103 (2) | 1 (5) | |||
| 9 | 1.5 × 103 ± 0.1 × 103 (2) | 6.0 × 103 ± 0.04 × 103 (1) | 2 (4) | |||
| 10 | Vaccine | 1.4 × 105 ± 0.1 × 105 (2) | 1.3 × 105 ± 0.1 × 105 (1) | 8 (2) | ||
| 11 | 9.2 × 104 ± 0.1 × 104 (2) | 6.8 × 104 ± 0.1 × 104 (1) | 8 (2) | |||
| 12 | 1.97 × 105 ± 0.1 × 105 (2) | 3.7 × 104 ± 0.1 × 104 (1) | 8 (2) | |||
| 13 | PBS | 1 | ND | ND | ND | |
| 14 | 3.4 × 105 ± 0.4 × 105 (2) | 1.38 × 105 ± 0.2 × 105 (1) | 9 (3) | |||
| 15 | 3.2 × 105 ± 0.3 × 105 (2) | 4.2 × 104 ± 0.4 × 104 (1) | 10 (3) | |||
| 16 | Bac-Con3N IFN-α and vaccine | 6 × 1010 | 3 | Neg | Neg | Neg |
| 17 | Neg | 2.1 ± 0.6 (5) | Neg | |||
| 18 | Neg | Neg | Neg | |||
| 19 | Vaccine | 4.24 × 104 ± 0.1 × 104 (2) | 4.2 × 104 ± 0.3 × 104 (2) | 10 (3) | ||
| 20 | 5.2 × 104 ± 0.1 × 104 (2) | 9.8 × 104 ± 0.1 × 104 (2) | 9 (3) | |||
| 21 | 3.7 × 104 ± 0.1 × 104 (2) | 3.2 × 105 ± 0.1 × 105 (2) | 9 (3) | |||
| 22 | Bac-Con3N IFN-α and vaccine | 7 | Neg | 1.8 × 101 ± 0.2 × 101 (5) | Neg | |
| 23 | Neg | 6.6 ± 3.6 (8) | Neg | |||
| 24 | Neg | Neg | Neg | |||
| 25 | Vaccine | 4.3 × 103 ± 0.2 × 103 (4) | 6.1 × 104 ± 0.1 × 104 (2) | 6 (3) | ||
| 26 | 4.72 × 103 ± 0.6 × 103 (4) | 9.7 × 103 ± 0.1 × 103 (2) | 6 (3) | |||
| 27 | 4.9 × 103 ± 0.2 × 103 (2) | 5.4 × 104 ± 0.1 × 104 (2) | 7 (3) | |||
| 28 | PBS | 3 | 6.4 × 104 ± 0.5 × 104 (2) | 5.9 × 104 ± 0.2 × 104 (2) | 10 (3) | |
| 29 | 1.7 × 105 ± 0.2 × 105 (2) | 5.7 × 105 ± 0.3 × 105 (2) | 9 (3) | |||
| 30 | 6.6 × 104 ± 0.9 × 104 (2) | 2.1 × 104 ± 0.2 × 104 (2) | 10 (3) | |||
a
dpv, days postvaccination; dpc, days postchallenge; Neg, no viral RNA and no clinical symptoms were detected; ND, not detected because pig 14 was dead at 1 dpc.
b
Data (copy numbers of FMDV per milliliter) represent the means ± SD from triplicate RT-qPCR measurements.