Table 1.
List and sequences of primers used for RT-qPCR analyses.
| Genes | Species | Primer sequences |
|---|---|---|
| Amelx (amelogenin) | m | 5′-AAGCATCCCTGAGCTTCAGA-3′ |
| 5′-ACTGGCATCATTGGTTGCTG-3′ | ||
| Ambn (ameloblastin) | m | 5′-AGCTGATAGCACCAGATGAG-3′ |
| 5′-GAACAGAGTTCCATAGGCCA-3′ | ||
| Enam (enamelin) | m | 5′-TCCTTGTTTTCCTGGGTCTG-3′ |
| 5′-ATCCATTGGGTACTGGTGGA-3′ | ||
| r | 5′-CATGTGGCCTCCGCCAGTCC-3′ | |
| 5′-GTCATCTGGGGGCGGGTCCT-3′ | ||
| Amtn (amelotin) | m | 5′-GGACAGCAACAGCTGCAA-3′ |
| 5′-TGTGAAGATTTGGGAGGCTAA-3′ | ||
| Mmp20 (matrix metalloproteinase 20) | m, r | 5′-CTGGGCCTGGGCCATTCCAC-3′ |
| 5′-CTGGTGATGGTGCTGGGCCG-3′ | ||
| Klk4 (kallikrein-related peptidase 4) | m | 5′-GCATCCGCAGTGGGTGCTGT-3′ |
| 5′-CACACTGCAGGAGGCTGGGC-3′ | ||
| Slc24a4 (solute carrier family 24, member 4) | m | 5′-ACGGAGATGTCGGTGTAGGA-3′ |
| 5′-CAATGGCACAGAAGGGTCGT-3′ | ||
| Slc26a4 (solute carrier family 26, member 4) | m | 5′-CGGAGCCCAAACAGGTGG-3′ |
| 5′-CCAAAGGCTCTCTTTCTTGAGC-3′ | ||
| Slc5a8 (solute carrier family 5, member 8) | m | 5′-ACGGTGGAACTGATAACCCG-3′ |
| 5′-GAAGCTTCACAAGCGAGTCC-3′ | ||
| AR (androgen receptor) | m | 5′-ACCTGACCTGGTTTTCAATGAGTATC-3′ |
| 5′-GTTATCTGGAGCCATCCAAACTCTT-3′ | ||
| (estrogen receptor alpha) | m | 5′-AAGAGAGTGCCAGGCTTTGG-3′ |
| 5′-ACGTAGCCAGCAACATGTCA-3′ | ||
| r | 5′-CCAGCTACAAACCAATGCACCATC-3′ | |
| 5′-GGTCTTTTCGTATCCCGCCTTTC-3′ | ||
| Gapdh (glyceraldehyde-3-phosphate dehydrogenase) | m, r | 5′-GACCCCTTCATTGACCTCAACTAC-3′ |
| 5′-AAGTTGTCATGGATGACCTTGGCC-3′ | ||
| Rs15 (ribosomal protein S15) | m, r | 5′-GGCTTGTAGGTGATGGAGAA-3′ |
| 5′-CTTCCGCAAGTTCACCTACC-3′ | ||
| Tbp1 (TATA-binding protein 1) | m | 5′-AGCTCTGGAATTGTACCGCA-3′ |
| 5′-AATCAACGCAGTTGTCCGTG-3′ | ||
| r | 5′-CACGAACAACTGCGTTGATC-3′ | |
| 5′-TTTTCTTGCTGCTAGTCTGGAT-3′ |
Note: m, mouse; r, rat; RT-qPCR, real-time quantitative polymerase chain reaction.