TABLE 3.
Relative activity of purified amylases towards different substrates
| Substrate | Relative activity (%)
|
|
|---|---|---|
| AmyA | AmyAΔ | |
| Soluble starch (potato) | 100a | 100a |
| Raw starch (corn) | 20 | 0 |
| Amylose (potato; type III) | 69 | 73 |
| Amylopectin (corn) | 137 | 112 |
| Glycogen | 69 | 9 |
| Pullulan | 0 | 0 |
| α-Cyclodextrin | 92 | 114 |
| β-Cyclodextrin | 0 | 0 |
| γ-Cyclodextrin | 0 | 0 |
Soluble potato starch was taken as the reference, giving 100% of enzyme activity.