TABLE 3.

Inhibition of laccase and immobilized laccase from T. hirsuta by various chemical substances

Laccase inhibitors	IC50 or % inhibition (mean ± SD)
	Laccase	Immobilized laccase
TGAa	500 μM ± 15 μM	600 μM ± 14 μM
NaN3	2.1 μM ± 0.1 μM	2.2 μM ± 0.1 μM
NaF	80 μM ± 2 μM	200 μM ± 8 μM
NaCl	50 mM ± 1 mM	85 mM ± 2 mM
NaBr	190 mM ± 8 mM	340 mM ± 14 mM
DDCa	1.2 mM ± 0.1 mM	4.2 mM ± 0.2 mM
EDTA	>200 mM	>200 mM
Dyeing auxiliaries (2 g liter−1)
Cibacel DBC (sequestering agent)	16.8% ± 0.7%	13.2% ± 0.5%
Univadine PA (anionic surfactant)	17.9% ± 0.9%	18.1% ± 1.2%
Tinegal MR (cationic surfactant)	5.2% ± 0.2%	6.4% ± 0.3%
Albegal FFA (wetting agent)	2.3% ± 0.2%	0.0%
Cibapon R (soaping agent)	2.1% ± 0.1%	0.0%

^aDCC, diethyldithiocarbamate; TGA, thioglycolic acid.