TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TGI | supE hsdΔ5thi Δ(lac-proAB)F′ [traD36 proAB+ lacqlacZΔM15] | 22 |
| L. plantarum CHL2 | Wild-type, gram-positive, ubiquitous homolactic acid bacterium | Charles Hansen's BioSystems, Horsholm, Denmark |
| L. plantarum NC8 | Plasmid-free strain | L. Axelsson, presented at the Fifth Symposium on Lactic Acid Bacteria, Veldhoven, The Netherlands, 1996. |
| Plasmids | ||
| pBluescript SK(−) | Apr ΔlacZ | Stratagene |
| pGID023 | Shuttle vector for E. coli and L. plantarum; derivative of pJDC9 containing the pE194 replication functions; used as an unstable integration vector; Emr | 24 |
| pBSK−FA | Apr; pBluescript SK(−) containing the 537-bp fragment (FA) of L. plantarum amplified by PCR with primers D1 and D2 | This study |
| pBSK−FAC | Apr; pBluescript SK(−) containing FA and the 497-bp (FC) fragment of L. plantarum amplified by PCR with primers D3 and D4 | This study |
| pGFAC | Emr; pGID023 containing the 1,034-bp FAC fragment | This study |
| pJDC9 | Emr; ΔlacZ | 11 |
| PJPDC1 | pJDC9 containing the 2.3-kb Sau3A fragment of L. plantarum CHL2 with the pdc gene | 7 |